A Controllable Solid-State Ru(bpy)32+ Electrochemiluminescence Film Based on Conformation Change of Ferrocene-Labeled DNA Molecular Beacon

详细信息    查看全文

• 作者：Xiaoying Wang ; Wen Yun ; Ping Dong ; Jingming Zhou ; Pingang He ; Yuzhi Fang
• 刊名：Langmuir
• 出版年：2008
• 出版时间：March 4, 2008
• 年：2008
• 卷：24
• 期：5
• 页码：2200 - 2205
• 全文大小：155K
• 年卷期：v.24,no.5(March 4, 2008)
• ISSN：1520-5827

文摘

A controllable solid-state electrochemiluminescence (ECL) film based on efficient and stable quenching of ECLof ruthenium(II) tris-(bipyridine) (Ru(bpy)₃²⁺) by oxidizing ferrocene (Fc) at the electrode is developed. The ECLintensity is correlated to the distance which is controlled by the conformation of the ferrocene-labeled DNA molecularbeacon (Fc-MB) between the Fc and Ru(bpy)₃²⁺ immobilized on the electrode. The conformation adjustment isconducted via complementary DNA hybridizing with the bases in the loop of the Fc-MB and changing the temperatureof the Fc-MB and the resultant double-stranded DNA (dsDNA). Those events all result in change of the ECL intensity.With such characteristics, the solid-state Ru(bpy)₃²⁺-ECL film has the potential to be applied to reagentless DNA ECLbiosensors and to calculate thermodynamic parameters of equilibrium constants of MB binding and the stem-loopformation.