Taking Advantage of Nonspecific Trypsin Cleavages for the Identification of Seed Storage Proteins in Cereals

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• 作者：Kjell Sergeant ; Carla Pinheiro ; Jean-Franois Hausman ; Cndido Pinto Ricardo ; Jenny Renaut
• 刊名：Journal of Proteome Research
• 出版年：2009
• 出版时间：June 5, 2009
• 年：2009
• 卷：8
• 期：6
• 页码：3182-3190
• 全文大小：215K
• 年卷期：v.8,no.6(June 5, 2009)
• ISSN：1535-3907

文摘

The lack of basic amino acids in seed storage proteins has resulted in the proposal to use chymotrypsin in their study. A comparative study of trypsin and chymotrypsin digestion initially confirmed this preference; however, reanalysis of the trypsin data set defining the specificity as ‘semitrypsin’ provided enough extra data to bridge the gap between both proteases. Rationale as to why numerous semitryptic peptides are observed in the study of these proteins is provided.