DNA Identification of Commercial Ginseng Samples
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- 作者：Jeremy J. Mihalov ; Ara Der Marderosian ; and James C. Pierce
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2000
- 出版时间：August 2000
- 年：2000
- 卷：48
- 期：8
- 页码：3744 - 3752
- 全文大小：170K
- 年卷期：v.48,no.8(August 2000)
- ISSN：1520-5118
文摘
An investigation was performed with the objective of developing a DNA-based protocol for theidentification of commercial samples of the herbal compound ginseng. There are currently two majorherbal products referred to as ginseng. They are Korean or Chinese ginseng (Panax ginseng) andAmerican ginseng (Panax quinquefolius). The market for ginseng in the United States is estimatedto be ~$300 million annually. Current tests for ginseng species identification rely on expert botanicalidentification of fresh plant/root specimens or on biochemical characterization of active and markercompounds (e.g., ginsenosides). For the determination of the feasibility of ginseng identification byDNA analysis, a strategy based on the direct DNA sequence analysis of the nuclear ribosomal internaltranscribed spacer region was developed. Other genetic tests included sequence analysis of thechloroplast ribulose 1,5-bisphosphate carboxylase large subunit gene and DNA fingerprinting bythe rapid amplification of polymorphic DNA technique. To confirm the results, each ginseng samplewas identified using high-performance liquid chromatography. All methods were successful indistinguishing American from Korean ginseng. In addition, the protocol was improved for theisolation of genomic and plastid DNA from commercial ginseng preparations by incorporating animpact homogenization step into the standard column chromatography purification procedure.Keywords: DNA testing; ginseng; Panax; herbal compounds; alternative medicine