Formation of Fibrinogen-Based Hydrogels Using Phototriggerable Diplasmalogen Liposomes
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- 作者：Zhi-Yi Zhang ; Pochi Shum ; Melinda Yates ; Phillip B. Messersmith ; and David H. Thompson
- 刊名：Bioconjugate Chemistry
- 出版年：2002
- 出版时间：May 2002
- 年：2002
- 卷：13
- 期：3
- 页码：640 - 646
- 全文大小：218K
- 年卷期：v.13,no.3(May 2002)
- ISSN：1520-4812
文摘
We report the triggered release of Ca2+ from liposomal compartments to induce rapid gelation ofprotein-based hydrogels. Phototriggerable liposomes were designed by entrapping CaCl2 withinliposomes composed of 38:57:5 diplasmenylcholine (DPPlsC):disteroylphosphatidylcholine (DSPC):bacteriochlorophyll (Bchl). These liposomes release >80% of their entrapped Ca2+ within 15 min whenirradiated at 800 nm (800 mW/cm2). A precursor solution, containing liposomes suspended in aqueoushuman fibrinogen and transglutaminase (TGase), remained fluid for several hours in the dark, butgelled rapidly when exposed to 800 nm excitation, as a result of photosensitized Ca2+ release andTG-induced fibrinogen cross-linking. TGase and hrFXIII activities, determined using a fluorimetricdansylcadaverine assay, were found to depend strongly on irradiation time and reaction temperature.SDS-PAGE of the photolyzed reaction mixture revealed that gelation arises from enzyme-catalyzedcross-linking of predominately the 
and 
chains of fibrinogen. This approach to the phototriggeredformation of protein hydrogels creates new opportunities for biomaterials applications in drug delivery,tissue engineering, and wound healing.
and chains of fibrinogen. This approach to the phototriggeredformation of protein hydrogels creates new opportunities for biomaterials applications in drug delivery,tissue engineering, and wound healing.