Topical Anti-inflammatory Activity of Eupatilin, A Lipophilic Flavonoid from Mountain Wormwood (Artemisia umbelliformis Lam.)
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- 作者：Anna Giangaspero ; Cristina Ponti ; Federica Pollastro ; Giorgia Del Favero ; Roberto Della Loggia ; Aurelia Tubaro ; Giovanni Appendino ; Silvio Sosa
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2009
- 出版时间：September 9, 2009
- 年：2009
- 卷：57
- 期：17
- 页码：7726-7730
- 全文大小：815K
- 年卷期：v.57,no.17(September 9, 2009)
- ISSN：1520-5118
文摘
Eupatilin (5,7-dihydroxy-3′,4′,6-trimethoxyflavone) is the major lipophilic flavonoid from Artemisia umbelliformis Lam. and Artemisia genipi Weber, two mountain wormwoods used for the production of the celebrated alpine liqueur genepy. The topical anti-inflammatory activity of eupatilin was investigated using the inhibition of the Croton-oil-induced dermatitis in the mouse ear as the end point. The oedematous response and the leukocyte infiltration were evaluated up to 48 h after the induction of phlogosis, comparing eupatilin with hydrocortisone and indomethacin as representatives of steroid and non-steroid anti-inflammatory drugs, respectively. At maximum development, eupatilin significantly reduced edema in a dose-dependent manner (ID50 = 0.28 μmol/cm2), showing an anti-inflammatory potency comparable to that of indomethacin (ID50 = 0.26 μmol/cm2) and only 1 order of magnitude lower than that of hydrocortisone (ID50 = 0.03 μmol/cm2). Within 48 h, eupatilin (0.30 μmol/cm2) caused a global inhibition of the oedematous response (42%) higher than that of an equimolar dose of indomethacin (18%) and fully comparable to that of 0.03 μmol/cm2 of hydrocortisone (55%). Moreover, the effect of eupatilin on the granulocytes infiltrate (32% inhibition) was similar to that of indomethacin (35% inhibition) and comparable to that of hydrocortisone (42% reduction), as confirmed by histological analysis. When our results are taken together, they show that eupatilin is endowed with potent in vivo topical anti-inflammatory activity, qualitatively similar to that of hydrocortisone and intermediate in terms of potency between those of steroid and non-steroid drugs.