Silver-Enhanced Colloidal Gold Electrochemical Stripping Detection of DNA Hybridization

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• 作者：Joseph Wang ; Ronen Polsky ; and Danke Xu
• 刊名：Langmuir
• 出版年：2001
• 出版时间：September 18, 2001
• 年：2001
• 卷：17
• 期：19
• 页码：5739 - 5741
• 全文大小：73K
• 年卷期：v.17,no.19(September 18, 2001)
• ISSN：1520-5827

文摘

We report on a novel method for detecting DNA hybridization, based on the precipitation of silver ongold nanoparticle tags and a subsequent electrochemical stripping detection of the dissolved silver. Suchcoupling of a nanoparticle-promoted silver precipitation with the remarkable sensitivity of stripping metalanalysis offers a dramatic enhancement of the hybridization response. An efficient magnetic isolation ofthe duplex is used for discriminating against nonhybridized DNA, including an excess of mismatchedoligonucleotides. The new silver-enhanced colloidal gold stripping detection strategy holds great promisefor the detection of DNA hybridization and represents an attractive alternative to indirect optical affinityassays of nucleic acids and other biomolecules.