Nucleic Acid-Functionalized Pt Nanoparticles: Catalytic Labels for the Amplified Electrochemical Detection of Biomolecules

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• 作者：Ronen Polsky ; Ron Gill ; Lubov Kaganovsky ; and Itamar Willner
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：April 1, 2006
• 年：2006
• 卷：78
• 期：7
• 页码：2268 - 2271
• 全文大小：351K
• 年卷期：v.78,no.7(April 1, 2006)
• ISSN：1520-6882

文摘

Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) actas catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPswith a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzedreduction of H₂O₂ (sensitivity limit, 1 × 10^-11 M).Similarly, the association of aptamer-functionalized Pt-NPs to a thrombin aptamer/thrombin complex associatedwith an electrode allowed the amplified, electrocatalyticdetection of thrombin with a sensitivity limit corresponding to 1 × 10^-9 M.