Partitioning of Cefazolin in Biocompatible Aqueous Biphasic Systems Based on Surfactant

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• 作者：Babak Madadi ; Gholamreza Pazuki ; Bahram Nasernejad
• 刊名：Journal of Chemical & Engineering Data
• 出版年：2013
• 出版时间：October 10, 2013
• 年：2013
• 卷：58
• 期：10
• 页码：2785-2792
• 全文大小：301K
• 年卷期：v.58,no.10(October 10, 2013)
• ISSN：1520-5134

文摘

In this research, promising aqueous two-phase systems (ATPS) containing polyoxyethylene (20) sorbitan monolaurate (Tween 20) as a quite biocompatible nonionic surfactant and different salts (K₂HPO₄, MgSO₄, and Na₃Citrate) were prepared and their extractive potential was evaluated. These ATPS possess top and bottom phases well-stocked in surfactant and salt respectively which can offer appealing characteristics such as low interfacial tension, low volatility, and ease of scaling-up. Ternary phase diagrams of ATPS consisting of Tween 20 and three different salts were obtained and the results were successfully fitted to the Merchuk correlation. The salting-out ability of salts were assessed. Also, the tie-line data were correlated with the Othmer-Tobias equation. Afterward, the partitioning behavior of cefazolin as a broad-spectrum antibiotic in the proposed ATPS was studied at three temperatures (298.15, 302.15, and 306.15 K) and the influences of Na₃Citrate and MgSO₄ on partition coefficients were justified by Hofmeister series. Furthermore, the effect of tie-line length on partitioning of cefazolin in ATPS was investigated. The experimental data of cefazolin partition coefficients indicated the proper potential of the Tween 20鈥揘a₃Citrate鈥搘ater biphasic system for the bioseparation process.