Proteomics of Fuchs鈥?Endothelial Corneal Dystrophy Support That the Extracellular Matrix of Descemet鈥檚 Membrane Is Disordered
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- 作者：Ebbe Toftgaard Poulsen ; Thomas F. Dyrlund ; Kasper Runager ; Carsten Scavenius ; Toke Peter Krogager ; Peter H酶jrup ; Ida B. Th酶gersen ; Kristian W. Sanggaard ; Henrik Vorum ; Jesper Hjortdal ; Jan J. Enghild
- 刊名：Journal of Proteome Research
- 出版年：2014
- 出版时间：November 7, 2014
- 年：2014
- 卷：13
- 期：11
- 页码：4659-4667
- 全文大小：269K
- ISSN：1535-3907
文摘
Fuchs鈥?endothelial corneal dystrophy (FECD) is a major corneal disorder affecting the innermost part of the cornea, leading to visual impairment. As the morphological changes in FECD are mainly observed in the extracellular matrix of the Descemet鈥檚 membrane/endothelial layer, we determined the protein profiles of diseased and control tissues using two relative quantitation MS methods. The first quantitation method, based on the areas of the extracted ion chromatograms, quantified the 51 and 48 most abundant proteins of the Descemet鈥檚 membrane/endothelial layer in patient and control tissues, respectively, of which 10 were significantly regulated. The results indicated that the level of type VIII collagen was unaltered even though the protein previously has been shown to be implicated in familial early-onset forms of the disease. Using the second relative quantitation method, iTRAQ, we identified 22 differentially regulated proteins, many of which are extracellular proteins known to be involved in proper assembly of the basement membrane in other tissues. In total, 26 differentially regulated proteins were identified, of which 6 proteins were regulated in both methods. These results support that the morphological changes observed in FECD are caused in part by an aberrant assembly of the extracellular matrix within the Descemet鈥檚 membrane/endothelial layer.<br>