Efficient and Selective Enzymatic Acylation Reaction: Separation of Furanosyl and Pyranosyl Nucleosides

详细信息    查看全文

• 作者：Jyotirmoy Maity ; Gaurav Shakya ; Sunil K. Singh ; Vasulinga T. Ravikumar ; Virinder S. Parmar ; Ashok K. Prasad
• 刊名：Journal of Organic Chemistry
• 出版年：2008
• 出版时间：July 18, 2008
• 年：2008
• 卷：73
• 期：14
• 页码：5629 - 5632
• 全文大小：142K
• 年卷期：v.73,no.14(July 18, 2008)
• ISSN：1520-6904

文摘

Candida antarctica lipase-B (CAL-B) immobilized on lewatite selectively acylated the primary hydroxyl group of the furanosyl nucleoside in a mixture of 1-(α-D-arabinofuranosyl)thymine and 1-(α-D-arabinopyranosyl)thymine. This selective biocatalytic acylation of furanosyl nucleoside has enabled us an easy separation of arabinofuranosyl thymine from an inseparable mixture with arabinopyranosyl thymine. The primary hydroxyl selective acylation methodology of arabinonucleoside has also been successfully used for the separation of 1-(β-D-xylofuranosyl)thymine and 1-(β-D-xylopyranosyl)thymine from a mixture of the two, which demonstrate the generality of the enzymatic methodology for separation of furanosyl and pyranosyl nucleosides.