Influence of 8-Methyl-nonenoic Acid on Capsaicin Biosynthesis in In-Vivo and In-Vitro Cell Cultures of Capsicum Spp.

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• 作者：Bellur Chayapathy Narasimha Prasad ; Harishchandra Bhaskar Gururaj ; Vinod Kumar ; Parvatam Giridhar ; Rangan Parimalan ; Ashwani Sharma ; and Gokare Ashwathnarayana Ravishankar
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：March 8, 2006
• 年：2006
• 卷：54
• 期：5
• 页码：1854 - 1859
• 全文大小：143K
• 年卷期：v.54,no.5(March 8, 2006)
• ISSN：1520-5118

文摘

Capsaicin is a bioactive molecule synthesized by enzymatic (putative capsaicin synthase) condensationof vanillylamine, a phenyl propanoid intermediate with 8-methyl-nonenoic acid, a fatty acid derivativefrom leucine/valine pathway. Analysis of levels of 8-methyl-nonenoic acid and phenyl propanoidintermediates in high, medium, and low pungent Capsicum genotypes revealed that the 8-methyl-nonenoic acid pool plays a crucial role in determining the efficacy of capsaicin levels. Cerulenin-mediated inhibition of 8-methyl-nonenoic acid synthesis decreased the capsaicin biosynthesis inCapsicum cell suspension cultures. Similarly amino oxy acetate inhibited vanillylamine synthesis butfailed to reduce capsaicin production. The mRNA transcript analysis of keto acyl synthase (KAS), acrucial enzyme involved in 8-methyl-nonenoic acid and an amino transferase (AMT), involved invanillylamine biosynthesis was studied. The mRNA transcript analysis revealed the progressivedevelopmental expression of KAS gene in the placenta during the ontogeny of the fruit, whereasAMT transcripts levels did not show significant differences. Hence, the study demonstrates theinfluence of 8-methyl-nonenoic acid and its possible regulatory role in capsaicin biosynthesis inCapsicum spp.Keywords: Capsicum spp.; capsaicin synthase; vanillylamine; 8-methyl-nonenoic acid; mRNAtranscripts; cerulenin; amino oxyacetate