Identification of Puerarin and Its Metabolites in Rats by Liquid Chromatography-Tandem Mass Spectrometry

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• 作者：Jeevan K. Prasain ; Kenneth Jones ; Nancy Brissie ; Ray Moore ; J. Michael Wyss ; and Stephen Barnes
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：June 16, 2004
• 年：2004
• 卷：52
• 期：12
• 页码：3708 - 3712
• 全文大小：96K
• 年卷期：v.52,no.12(June 16, 2004)
• ISSN：1520-5118

文摘

Puerarin (daidzein-8-C-glucoside) is the major bioactive isoflavone of kudzu root (the root of Puerarialobata). Its metabolic fate, however, is not well-known. In this study, a sensitive and specific LC-ESI-MS/MS method for the determination of puerarin and its metabolites daidzein, dihydrodaidzein,and equol was developed for their analysis in biological samples. Two new metabolites of puerarin,mono- and dihydroxylated derivatives, were detected in the urine and feces of rats after oraladministration. The persistence of puerarin in blood and urine as the principal metabolic form for theperiod of 4-72 h after oral administration suggested that puerarin is rapidly absorbed from the intestinewithout metabolism. Its presence in organs such as the brain suggests that this glucoside may entertissues by specific transport pathways. Study of these metabolites may provide further understandingof the health beneficial effects of puerarin in kudzu dietary supplements.Keywords: Puerarin; metabolites; C-glucoside; kudzu; LC-MS/MS; rats