Immobilization of Iron Oxide Magnetic Nanoparticles for Enhancement of Vessel Wall Magnetic Resonance Imaging—An Ex Vivo Feasibility Study

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• 作者：Binh Thai Nguyen ; Praveen Kumar Vemula ; Dimitrios Mitsouras ; Peng Yu ; Ming Tao ; Christina Campagna ; Robert V. Mulkern ; Frank J. Rybicki ; Jeffrey M. Karp ; C. Keith Ozaki
• 刊名：Bioconjugate Chemistry
• 出版年：2010
• 出版时间：August 18, 2010
• 年：2010
• 卷：21
• 期：8
• 页码：1408-1412
• 全文大小：231K
• 年卷期：v.21,no.8(August 18, 2010)
• ISSN：1520-4812

文摘

Emerging data supports a role for negative wall remodeling in the failure of vascular interventions such as vein grafts, yet clinicians/researchers currently lack the ability to temporally/efficiently investigate adventitial surface topography/total vascular wall anatomy in vivo. We established a strategy of immobilizing commercially available iron oxide magnetic nanoparticles (Fe-NPs) onto the surface of human vein conduits to facilitate high-throughput total vascular wall demarcation with magnetic resonance (MR). Binding of activated Fe-NPs to amine groups on the surface of the veins induced a thin layer of negative contrast that differentiated the adventitia from surrounding saline signal in all MR images, enabling delineation of total wall anatomy; this was not possible in simultaneously imaged unlabeled control veins. Under the conditions of this ex vivo experiment, stable covalent binding of Fe-NPs can be achieved (dose-dependent) on human vein surface for MR detection, suggesting a potential strategy for enhancing the ability of MRI to investigate total wall adaptation and remodeling in vein graft failure.