文摘
Recently, transgenic potato plants were created with overexpression of the 14-3-3 protein derivedfrom Cucurbita pepo. Detailed analysis of those plants suggested that the function of the isolated14-3-3 isoform is in the control of carbohydrate and lipid metabolism in the plants. 14-3-3 proteinoverexpression gave rise to an increase in soluble sugar and catecholamine contents in both leavesand tubers. It is proposed that 14-3-3 protein affects carbohydrate metabolism in potato plants viaregulation of catecholamine synthesis. Furthermore, genetically modified potato tubers with 14-3-3 protein overexpression showed changes in lipid content and composition. The transgenic potatotubers contained 69% more total fat compared to the wild-type plant. Separation of tuber lipidsinto polar and nonpolar fractions revealed that the transgenic potato tubers contained almost 3times more nonpolar lipids than the control. Analysis of fatty acid composition, conducted by themeans of gas chromatography, showed that linoleic acid was the main fatty acid present in thetubers of both modified and control potato plants. In the nonpolar fraction of the fat of the transgenictubers the unsaturated fatty acids exhibited a higher participation in the sum of all fatty acids.Keywords: 14-3-3 protein; transgenic plant; catecholamines; carbohydrate metabolism; lipidanalysis; Solanum tuberosum