Lipase-Mediated Purification of Methyl Nonactate, an Important Natural Product Building Block for Diversity-Oriented Synthesis

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• 作者：Jennifer M. Dinges ; Bradley A. Bessette Jr. ; James E. Cox ; Casey R. Redder ; Nigel D. Priestley
• 刊名：Biotechnology Progress
• 出版年：2006
• 出版时间：October 2006
• 年：2006
• 卷：22
• 期：5
• 页码：1354 - 1357
• 全文大小：51K
• 年卷期：v.22,no.5(October 2006)
• ISSN：1520-6033

文摘

Methyl nonactate is a valuable starting material for the production of natural product-like combinatorial libraries and ketide amino acids, a series of novel conformationally constrained aminoacid analogues. Fermentation of Streptomyces griseus generates high titers of macrotetrolideantibiotics from which methyl nonactate can be generated by methanolysis. Unfortunately, thisapproach generates mixtures of homologues that are not economically separable. We report aspecific lipase-mediated hydrolysis of nonactate derivatives that discriminates between homologues and is the foundation of an economically tractable preparation of methyl nonactate inscale.