Characterization of Single-Isomer, Heptasulfated -Cyclodextrins by Electrospray Ionization Mass Spectrometry and Indi
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- 作者：Pavel V. Bondarenko ; Barbara Wolf ; Hong Cai ; J. Bryan Vincent ; Ronald D. Macfarlane ; and Gyula Vigh
- 刊名：Analytical Chemistry
- 出版年：1998
- 出版时间：July 15, 1998
- 年：1998
- 卷：70
- 期：14
- 页码：3042 - 3045
- 全文大小：56K
- 年卷期：v.70,no.14(July 15, 1998)
- ISSN：1520-6882
文摘
The molecular weight, the purity, and the degree ofsubstitution for several newly synthesized single-isomer,heptasulfated 
-cyclodextrin derivatives,heptakis-6-sulfato--cyclodextrin,heptakis(2,3-diacetyl-6-sulfato)--cyclodextrin, andheptakis(2,3-dimethyl-6-sulfato)--cyclodextrin, were determined by electrospray ionization massspectrometry and indirect UV detection capillary electrophoresis. The main fragmentation mechanism of thesesingle-isomer, fully sulfated cyclodextrins involves dissociation of the sulfate groups. The extent ofdesulfationincreases with the skimmer-collimator voltage bias andthe number of charges on the electrospray ions. Byreducing the skimmer-collimator voltage bias, the lossof sulfate groups could be reduced, though not completelyeliminated, for the doubly charged molecular ions.Thus,ESI-MS can be used to confirm the degree of substitutionfor the most sulfated isomer, but not to determine theexact concentration distribution for the lower substitutedisomers in a mixture.
-cyclodextrin derivatives,heptakis-6-sulfato--cyclodextrin,heptakis(2,3-diacetyl-6-sulfato)--cyclodextrin, andheptakis(2,3-dimethyl-6-sulfato)--cyclodextrin, were determined by electrospray ionization massspectrometry and indirect UV detection capillary electrophoresis. The main fragmentation mechanism of thesesingle-isomer, fully sulfated cyclodextrins involves dissociation of the sulfate groups. The extent ofdesulfationincreases with the skimmer-collimator voltage bias andthe number of charges on the electrospray ions. Byreducing the skimmer-collimator voltage bias, the lossof sulfate groups could be reduced, though not completelyeliminated, for the doubly charged molecular ions.Thus,ESI-MS can be used to confirm the degree of substitutionfor the most sulfated isomer, but not to determine theexact concentration distribution for the lower substitutedisomers in a mixture.