Absolute Transcript Expression Signatures of Cyp and Gst Genes in Mus spretus to Detect Environmental Contamination

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• 作者：Julia Ruiz-laguna ; Nieves Abril ; Tamara GarcÍ ; a-barrera ; Jose-luis GÓ ; mez-ariza ; Juan LÓ ; pez-barea ; and Carmen Pueyo
• 刊名：Environmental Science & Technology
• 出版年：2006
• 出版时间：June 1, 2006
• 年：2006
• 卷：40
• 期：11
• 页码：3646 - 3652
• 全文大小：406K
• 年卷期：v.40,no.11(June 1, 2006)
• ISSN：1520-5851

文摘

We evaluated whether quantitation of mRNA molecules ofkey genes is a reliable biomonitoring end-point. Weexamined the Mus spretus expression levels of 19 transcriptsencoding different cytochrome-P450s and glutathionetransferases. Mice dwelling at the Doñana BiologicalReserve were compared to those from an industrial settlement(PS). Metal biomonitoring indicated that PS animalssustained a heavier pollutant burden than those from thereference site. Transcript quantitations showed thefollowing: (i) gender-related differences in the expressionof most Cyp and Gst genes; (ii) one PS female displayingmuch smaller/larger transcript amounts than the remainingfemales; (iii) the concomitant up-regulation of Cyp1a2,Cyp2a5, Cyp2e1, Cyp4a10, Gsta1, Gsta2, Gstm1, and Gstm2mRNAs in liver of PS males; and (iv) outstanding qualitativeand quantitative differences between the hepatic expressionsignature of PS males and that promoted by paraquat.We conclude that (i) absolute amounts of transcripts encodingbiotransformation enzymes are more potent biomarkersin males than in females, and in liver than in kidney; (ii)individual quantitations prevent biased interpretations byspecimens with abnormal expression levels; and (iii) transcriptexpression signature of PS males is consistent withexposure to a complex profile of organic pollutants, otherthan oxidative stressors.