Synthesis and Biophysical Properties of Constrained d-Altritol Nucleic Acids (cANA)

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• 作者：Michael T. Migawa ; Thazha P. Prakash ; Guillermo Vasquez ; Punit P. Seth ; Eric E. Swayze
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：September 6, 2013
• 年：2013
• 卷：15
• 期：17
• 页码：4316-4319
• 全文大小：244K
• 年卷期：v.15,no.17(September 6, 2013)
• ISSN：1523-7052

文摘

The first synthesis of constrained altritol nucleic acids (cANA) containing antisense oligonucleotides (ASOs) was carried out to ascertain how conformationally restricting the d-altritol backbone-containing ASO (Me-ANA) would affect their ability to form duplexes with RNA. It was found that the thermal stability was reduced (cANA/RNA 鈭?.1 掳C/modification) compared to DNA/RNA, suggesting the constrained system results in a small destabilizing perturbation in the duplex structure.