A Constrained Tricyclic Nucleic Acid Analogue of 伪-l-LNA: Investigating the Effects of Dual Conformational Restriction on Duplex Thermal Stability
详细信息 查看全文
- 作者：Stephen Hanessian ; Jernej Wagger ; Bradley L. Merner ; Robert D. Giacometti ; Michael E. 脴stergaard ; Eric E. Swayze ; Punit P. Seth
- 刊名：Journal of Organic Chemistry
- 出版年：2013
- 出版时间：September 20, 2013
- 年：2013
- 卷：78
- 期：18
- 页码：9064-9075
- 全文大小：539K
- 年卷期：v.78,no.18(September 20, 2013)
- ISSN：1520-6904
文摘
A constrained tricyclic analogue of 伪-l-LNA (2), which contains dual modes of conformational restriction about the ribose sugar moiety, has been synthesized and characterized by X-ray crystallography. Thermal denaturation experiments of oligonucleotide sequences containing this tricyclic 伪-l-LNA analogue (伪-l-TriNA 2, 5) indicate that this modification is moderately stabilizing when paired with complementary DNA and RNA, but less stabilizing than both 伪-l-LNA (2) and 伪-l-TriNA 1 (4).