In Vivo Determination of the Molecular Composition of Artery Wall by Intravascular Raman Spectroscopy

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• 作者：Hendrik P. Buschman ; Eric T. Marple ; Michael L. Wach ; Bob Bennett ; Tom C. Bakker Schut ; Hajo A. Bruining ; Albert V. Bruschke ; Arnoud van der Laarse ; and Gerwin J. Puppels
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：August 15, 2000
• 年：2000
• 卷：72
• 期：16
• 页码：3771 - 3775
• 全文大小：169K
• 年卷期：v.72,no.16(August 15, 2000)
• ISSN：1520-6882

文摘

Atherosclerotic plaque vulnerability is suggested to bedetermined by its chemical composition. However, atpresent there are no in vivo techniques available that canadequately type atherosclerotic plaques in terms of chemical composition. Previous in vitro experiments haveshown that Raman spectroscopy can provide such information in great detail. Here we present the results of invitro and in vivo intravascular Raman spectroscopicexperiments, in which dedicated, miniaturized fiber-opticprobes were used to illuminate the blood vessel wall andto collect Raman scattered light. The results make clearthat an important hurdle to clinical application of Ramanspectroscopy in atherosclerosis has been overcome,namely, the ability to obtain in vivo intravascular Ramanspectra of high quality. Of equal importance is the findingthat the in vivo intravascular Raman signal obtained froma blood vessel is a simple summation of signal contributions of the blood vessel wall and of blood. It means thatdetailed information about the chemical composition ofa blood vessel wall can be obtained by adapting a multipleleast-squares fitting method, which was developed previously for the analysis of in vitro spectra, to account forsignal contributions of blood.