Algorithms for Validating Chiral Properties of Insulins

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• 作者：Neil Purdie ; Dennis W. Province ; Thomas P. Layloff ; and Moheb M. Nasr
• 刊名：Analytical Chemistry
• 出版年：1999
• 出版时间：August 15, 1999
• 年：1999
• 卷：71
• 期：16
• 页码：3341 - 3346
• 全文大小：58K
• 年卷期：v.71,no.16(August 15, 1999)
• ISSN：1520-6882

文摘

The combination of chiral ligand exchange on Cu(II)complexes in aqueous base with circular dichroism spectropolarimetric detection provides excellent avenues tovalidate the chirality properties of oligopeptides andproteins. The method is quick and simple and has thepotential for development into an automated, routineprocedure for quality control applications. Target analytesused for this first study of a protein system are human,porcine, and bovine insulins prepared by different procedures and obtained from different sources, productionlots, and manufacturers. The analytical specificity of thetest makes the method a potentially useful technique forvalidating the chirality properties of many peptide andprotein forms.