Nanopatterned Cadmium Selenide Langmuir鈥揃lodgett Platform for Leukemia Detection
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- 作者：Aditya Sharma ; Chandra M. Pandey ; Zimple Matharu ; Udit Soni ; Sameer Sapra ; Gajjala Sumana ; Manoj K. Pandey ; Tathagat Chatterjee ; Bansi D. Malhotra
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：April 3, 2012
- 年：2012
- 卷：84
- 期：7
- 页码：3082-3089
- 全文大小：589K
- 年卷期：v.84,no.7(April 3, 2012)
- ISSN：1520-6882
文摘
We present results of the studies relating to preparation of Langmuir鈥揃lodgett (LB) monolayers of tri-n-octylphosphine oxide-capped cadmium selenide quantum dots (QCdSe) onto indium鈥搕in oxide (ITO) coated glass substrate. The monolayer behavior has been studied at the air鈥搘ater interface under various subphase conditions. This nanopatterned platform has been explored to fabricate an electrochemical DNA biosensor for detection of chronic myelogenous leukemia (CML) by covalently immobilizing the thiol-terminated oligonucleotide probe sequence via a displacement reaction. The results of electrochemical response studies reveal that this biosensor can detect target DNA in the range of 10鈥? to 10鈥?4 M within 120 s, has a shelf life of 2 months, and can be used about 8 times. Further, this nucleic acid sensor has been found to distinguish the CML-positive and the control negative clinical patient samples.