Binding-Directed Energy Transfer of Conjugated Polymer Materials for Dual-Color Imaging of Cell Membrane

详细信息    查看全文

• 作者：Qianling Cui ; Xiaoyu Wang ; Yu Yang ; Shengliang Li ; Lidong Li ; Shu Wang
• 刊名：Chemistry of Materials
• 出版年：2016
• 出版时间：July 12, 2016
• 年：2016
• 卷：28
• 期：13
• 页码：4661-4669
• 全文大小：532K
• 年卷期：0
• ISSN：1520-5002

文摘

Binding of biomolecules or probes to the plasma membrane is of great importance for investigations of cell morphology and various biological processes. Herein, a water-soluble conjugated polymer is designed as a membrane probe. The probe shows a strong affinity toward lipid membranes owing to the high charge density from abundant imidazolium moieties together with the moderate rigidity and hydrophobicity derived from the conjugated backbone. Upon binding with a membrane, the interchain FRET of the probe was substantially enhanced, which resulted in the emission of both blue and red fluorescence. This is favorable for dual-color imaging. Finally, cellular experiments demonstrate the excellent performance of this macromolecular probe on stable binding with cell membranes without the appearance of cell endocytocysis even after a long retention time.