Efficient Conversion of Inulin to Inulooligosaccharides through Endoinulinase from Aspergillus niger

详细信息    查看全文

• 作者：Yanbing Xu ; Zhaojuan Zheng ; Qianqian Xu ; Qiang Yong ; Jia Ouyang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：March 30, 2016
• 年：2016
• 卷：64
• 期：12
• 页码：2612-2618
• 全文大小：575K
• ISSN：1520-5118

文摘

Inulooligosaccharides (IOS) represent an important class of oligosaccharides at industrial scale. An efficient conversion of inulin to IOS through endoinulinase from Aspergillus niger is presented. A 1482 bp codon optimized gene fragment encoding endoinulinase from A. niger DSM 2466 was cloned into pPIC9K vector and was transformed into Pichia pastoris KM71. Maximum activity of the recombinant endoinulinase, 858 U/mL, was obtained at 120 h of the high cell density fermentation process. The optimal conditions for inulin hydrolysis using the recombinant endoinulinase were investigated. IOS were harvested with a high concentration of 365.1 g/L and high yield up to 91.3%. IOS with different degrees of polymerization (DP, mainly DP 3–6) were distributed in the final reaction products.