Extraction and Analysis of Auxins in Plants Using Dispersive Liquid−Liquid Microextraction Followed by High-Performance Liquid Chromatography with Fluorescence Detection
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- 作者：Qiaomei Lu ; Lihui Chen ; Minghua Lu ; Guonan Chen ; Lan Zhang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：March 10, 2010
- 年：2010
- 卷：58
- 期：5
- 页码：2763-2770
- 全文大小：805K
- 年卷期：v.58,no.5(March 10, 2010)
- ISSN：1520-5118
文摘
Auxin plays an important role in cell differentiation, apical dominance, and tropism in plants. A new method based on dispersive liquid−liquid microextraction (DLLME) combined with high-performance liquid chromatography−fluorescence detection (HPLC-FLD) has been established to detect auxin. A mixture of CHCl3 (extraction solvent) and acetone (disperser solvent) was injected quickly into a sample solution with desired salt concentration and pH value, and then a cloudy solution consisting of many dispersed fine droplets of CHCl3 was formed. After centrifugation, the sedimented phase was withdrawn and directly analyzed by HPLC-FLD. Under optimal conditions, four auxins were baseline separated within 3.5 min, with the minimal limit of detection of 0.02 ng mL−1 and coefficient correlations in the range of 0.9980−0.9995. This simple method was successfully applied to real sample analysis. Experimental results showed that DLLME was a high-performance and powerful preconcentration method to extract and enrich related plant auxin.