Size-Dependent Cell Uptake of Protein-Coated Graphene Oxide Nanosheets
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- 作者：Qingxin Mu ; Gaoxing Su ; Liwen Li ; Ben O. Gilbertson ; Lam H. Yu ; Qiu Zhang ; Ya-Ping Sun ; Bing Yan
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2012
- 出版时间：April 25, 2012
- 年：2012
- 卷：4
- 期：4
- 页码：2259-2266
- 全文大小：488K
- 年卷期：v.4,no.4(April 25, 2012)
- ISSN：1944-8252
文摘
As an emerging applied material, graphene has shown tremendous application potential in many fields, including biomedicine. However, the biological behavior of these nanosheets, especially their interactions with cells, is not well understood. Here, we report our findings about the cell surface adhesion, subcellular locations, and size-dependent uptake mechanisms of protein-coated graphene oxide nanosheets (PCGO). Small nanosheets enter cells mainly through clathrin-mediated endocytosis, and the increase of graphene size enhances phagocytotic uptake of the nanosheets. These findings will facilitate biomedical and toxicologic studies of graphenes and provide fundamental understanding of interactions at the interface of two-dimensional nanostructures and biological systems.