High-Speed, Whole-Column Fluorescence Imaging Detection for Isoelectric Focusing on a Microchip Using an Organic Light Emitting Diode as Light Source
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- 作者：Bo Yao ; Huihua Yang ; Qionglin Liang ; Guoan Luo ; Liduo Wang ; Kangning Ren ; Yudi Gao ; Yiming Wang ; Yong Qiu
- 刊名：Analytical Chemistry
- 出版年：2006
- 出版时间：August 15, 2006
- 年：2006
- 卷：78
- 期：16
- 页码：5845 - 5850
- 全文大小：246K
- 年卷期：v.78,no.16(August 15, 2006)
- ISSN：1520-6882
文摘
An integrated and simplified microfluidic device using a250 
m × 1-4 cm of organic light emitting diode (OLED)array as a two-dimensional light source for single-channeland multichannel whole-column imaging detection wasdeveloped. This fluorescence detection system was usedfor isoelectric focusing (IEF) of R-phycoerythrin in amicrochip. The IEF conditions were optimized, and thetotal analysis time was extremely reduced to 30 s for2-cm-long microchannels at 700 V/cm of electric fieldstrength without the presence of electroosmotic flow. Thecompression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when1% hydroxylpropylmethyl cellulose in 2% ampholyte wasused as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was ~0.6 g/mLor 45 pg at 75 nL/column injection of R-phycoerythrin.This OLED-induced fluorescence detection system forWCID provides a high-speed IEF technique with quantitative ability and the potential for high integration andthroughput microchip systems.
m × 1-4 cm of organic light emitting diode (OLED)array as a two-dimensional light source for single-channeland multichannel whole-column imaging detection wasdeveloped. This fluorescence detection system was usedfor isoelectric focusing (IEF) of R-phycoerythrin in amicrochip. The IEF conditions were optimized, and thetotal analysis time was extremely reduced to 30 s for2-cm-long microchannels at 700 V/cm of electric fieldstrength without the presence of electroosmotic flow. Thecompression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when1% hydroxylpropylmethyl cellulose in 2% ampholyte wasused as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was ~0.6 g/mLor 45 pg at 75 nL/column injection of R-phycoerythrin.This OLED-induced fluorescence detection system forWCID provides a high-speed IEF technique with quantitative ability and the potential for high integration andthroughput microchip systems.