Structure of the N-Terminal RNA-Binding Domain of the SARS CoV Nucleocapsid Protein
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- 作者：Qiulong Huang ; Liping Yu ; Andrew M. Petros ; Angelo Gunasekera ; Zhihong Liu ; Nan Xu ; Philip Hajduk ; Jamey Mack ; Stephen W. Fesik ; and Edward T. Olejniczak
- 刊名：Biochemistry
- 出版年：2004
- 出版时间：May 25, 2004
- 年：2004
- 卷：43
- 期：20
- 页码：6059 - 6063
- 全文大小：186K
- 年卷期：v.43,no.20(May 25, 2004)
- ISSN：1520-4995
文摘
The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family ofviruses. Its virion encodes several proteins including a replicase and four structural proteins. Here wedescribe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV)nucleocapsid protein. The protein consists of a five-stranded 
sheet with a folding topology distinctfrom other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction betweena flexible, positively charged hairpin and the core structure. NMR-based screening was used to identifylow molecular weight compounds that bind to this site.
sheet with a folding topology distinctfrom other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction betweena flexible, positively charged hairpin and the core structure. NMR-based screening was used to identifylow molecular weight compounds that bind to this site.