Assessment of Analytical Reproducibility of 1H NMR Spectroscopy Based Metabonomics for Large-Scale Epidemiological Research: the INTERMAP Study

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• 作者：Marc-Emmanuel Dumas ; Elaine C. Maibaum ; Claire Teague ; Hirotsugu Ueshima ; Beifan Zhou ; John C. Lindon ; Jeremy K. Nicholson ; Jeremiah Stamler ; Paul Elliott ; Queenie Chan ; and Elaine Holmes
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：April 1, 2006
• 年：2006
• 卷：78
• 期：7
• 页码：2199 - 2208
• 全文大小：402K
• 年卷期：v.78,no.7(April 1, 2006)
• ISSN：1520-6882

文摘

Large-scale population phenotyping for molecular epidemiological studies is subject to all the usual criteria ofanalytical chemistry. As part of a major phenotypinginvestigation we have used high-resolution ¹H NMRspectroscopy to characterize 24-h urine specimens obtained from population samples in Aito Town, Japan (n= 259), Chicago, IL (n = 315), and Guangxi, China (n =278). We have investigated analytical reproducibility,urine specimen storage procedures, interinstrument variability, and split specimen detection. Our data show thatthe multivariate analytical reproducibility of the NMRscreening platform was >98% and that most classificationerrors were due to urine specimen handling inhomogeneity. Differences in metabolite profiles were then assessed for Aito Town, Chicago, and Guangxi populationsamples; novel combinations of biomarkers were detectedthat separated the population samples. These cross-population differences in urinary metabolites could berelated to genetic, dietary, and gut microbial factors.