Mannoside Glycolipid Conjugates Display Anti-inflammatory Activity by Inhibition of Toll-like Receptor-4 Mediated Cell Activation

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• 作者：Vincent Flacher ; Patrick Neuberg ; Floriane Point ; Fran莽ois Daubeuf ; Quentin Muller ; David Sigwalt ; Jean-Daniel Fauny ; Jean-Serge Remy ; Nelly Frossard ; Alain Wagner ; Christopher G. Mueller ; Evelyne Schaeffer
• 刊名：ACS Chemical Biology
• 出版年：2015
• 出版时间：December 18, 2015
• 年：2015
• 卷：10
• 期：12
• 页码：2697-2705
• 全文大小：497K
• ISSN：1554-8937

文摘

Inhibition of excessive Toll-like receptor 4 (TLR4) signaling is a therapeutic approach pursued for many inflammatory diseases. We report that Mannoside Glycolipid Conjugates (MGCs) selectively blocked TLR4-mediated activation of human monocytes and monocyte-derived dendritic cells (DCs) by lipopolysaccharide (LPS). They potently suppressed pro-inflammatory cytokine secretion and maturation of DCs exposed to LPS, leading to impaired T cell stimulation. MGCs did not interfere with LPS and could act in a delayed manner, hours after LPS stimulation. Their inhibitory action required both the sugar heads and the lipid chain, although the nature of the sugar and the structure of the lipid tail could be modified. They blocked early signaling events at the cell membrane, enhanced internalization of CD14 receptors, and prevented colocalization of CD14 and TLR4, thereby abolishing NF-魏B nuclear translocation. When the best lead conjugate was tested in a mouse model of LPS-induced acute lung inflammation, it displayed an anti-inflammatory action by suppressing the recruitment of neutrophils. Thus, MGCs could serve as promising leads for the development of selective TLR4 antagonistic agents for inflammatory diseases.