Molecular Cloning and Characterization of Two 12 kDa FK506-Binding Protein Genes in the Chinese Oak Silkworm, Antheraea pernyi
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- 作者：Mo Chen ; Miao-Miao Chen ; Rui Yao ; Yan Li ; Huan Wang ; Yu-Ping Li ; Yan-Qun Liu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：May 15, 2013
- 年：2013
- 卷：61
- 期：19
- 页码：4599-4605
- 全文大小：548K
- 年卷期：v.61,no.19(May 15, 2013)
- ISSN：1520-5118
文摘
Two 12 kDa FK506-binding protein (FKBP12) genes were isolated and characterized from Chinese oak silkworm Antheraea pernyi, an important agricultural and edible insect, designated ApFKBP12 A and B, respectively. Both ApFKBP12 A and B contained 108 amino acids with 82% sequence identity. Phylogenetic analysis showed that FKBP12 B sequences of A. pernyi, Bombyx mori, and Danaus plexippus were clearly separated from FKBP12 A sequences of these three species, suggesting that insect FKBP12 A and B may have been evolving independently. RT-PCR analyses revealed that two ApFKBP12 genes were expressed during the four developmental stages and in all tested tissues, and that the mRNA expression level of the ApFKBP12 A gene was significantly higher than that of the ApFKBP12 B gene. After heat shock treatment, expressions of the two FKBP12 genes were up-regulated, but at different time points. The results suggested that each paralogue of the FKBP12 genes may play a distinct functional role in the development of A. pernyi.