Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Micellar Electrokinetic Chromatography and Near-Field Thermal Lens Detection
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- 作者：Natalia Y. Ragozina ; Michael Pü ; tz ; Stefan Heissler ; Werner Faubel ; and Ute Pyell
- 刊名：Analytical Chemistry
- 出版年：2004
- 出版时间：July 1, 2004
- 年：2004
- 卷：76
- 期：13
- 页码：3804 - 3809
- 全文大小：95K
- 年卷期：v.76,no.13(July 1, 2004)
- ISSN：1520-6882
文摘
A method employing micellar electrokinetic chromatography in combination with near-field thermal lens detection (NF-TLD) was developed for the rapid simultaneousdetermination of etoposide phosphate and etoposide inhuman blood plasma, taking teniposide as internal standard. The method developed allows the baseline separation of the solutes of interest from each other and frompotential interfering matrix constituents within 4 min. TheNF-TLD device employed permits detection of solutesabsorbing electromagnetic radiation at 
= 257 nm infused-silica capillaries with 75-
m i.d. via the near-fieldthermal lens effect with LODs of 100 g L-1 for etoposidephosphate and 170 g L-1 for etoposide. Comparison ofthe performance of this detector to the performance of acommercial absorption spectrometric detector working at = 257 nm showed a substantial improvement in detection limits (up to 60-fold improvement) for the near-fieldthermal lens detector.
= 257 nm infused-silica capillaries with 75-m i.d. via the near-fieldthermal lens effect with LODs of 100 g L-1 for etoposidephosphate and 170 g L-1 for etoposide. Comparison ofthe performance of this detector to the performance of acommercial absorption spectrometric detector working at = 257 nm showed a substantial improvement in detection limits (up to 60-fold improvement) for the near-fieldthermal lens detector.