Novel Hydrogels via Click Chemistry: Synthesis and Potential Biomedical Applications

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• 作者：Vittorio Crescenzi ; Lisa Cornelio ; Chiara Di Meo ; Stefania Nardecchia ; Raffaele Lamanna
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：8
• 期：6
• 页码：1844 - 1850
• 全文大小：723K
• 年卷期：v.8,no.6(June 2007)
• ISSN：1526-4602

文摘

A novel procedure for the in situ rapid chemical gelation of aqueous solutions of hyaluronan has been employed.In brief, water-soluble polysaccharide derivatives bearing side chains endowed with either azide or alkyne terminalfunctionality have been prepared. When the latter two types of derivatives are mixed together in aqueous solutionthey give rise to a 1,3-dipolar cycloaddition reaction resulting in fast gelation (in the presence of catalytic amountsof Cu(I)) at room temperature. Gel formation has been characterized rheologically and could also be followedqualitatively by means of IR spectroscopy. The resulting gels have been studied in terms of swelling propertiesand, in particular, NMR spectral features. Carrying out the gelation process in aqueous solutions of benzidamineand doxorubicin, respectively, the polysaccharide networks acted as drug reservoirs. The doxorubicin releaseresulted in well controllable acting upon the gels degree of cross-linking. Finally, formation of the click-gelsusing aqueous suspensions of Saccharomices cerevisiae yeast cells allowed the obtainment of scaffolds insidewhich cells were homogeneously distributed and smoothly adhered to the inner pores surfaces, according to SEManalysis. After 24 h about 60% of the entrapped cells exhibited proliferating activity. Click-gels prepared asdetailed herein do have a number of positive features that make them, in perspective, materials of choice for drugrelease and tissue engineering manipulations.