Resonance Energy Transfer Improves the Biological Function of Bacteriorhodopsin within a Hybrid Material Built from Purple Membranes and Semiconductor Quantum Dots
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- 作者：Aliaksandra Rakovich ; Alyona Sukhanova ; Nicolas Bouchonville ; Evgeniy Lukashev ; Vladimir Oleinikov ; Mikhail Artemyev ; Vladimir Lesnyak ; Nikolai Gaponik ; Michael Molinari ; Michel Troyon ; Yury P. Rakovi
- 刊名：Nano Letters
- 出版年：2010
- 出版时间：July 14, 2010
- 年：2010
- 卷：10
- 期：7
- 页码：2640-2648
- 全文大小：670K
- 年卷期：v.10,no.7(July 14, 2010)
- ISSN：1530-6992
文摘
Purple membrane (PM) from bacteria Halobacterium salinarum contains a photochromic protein bacteriorhodopsin (bR) arranged in a 2D hexagonal nanocrystalline lattice (Figure 1). Absorption of light by the protein-bound chromophore retinal results in pumping the protons through the PM creating an electrochemical gradient which is then used by the ATPases to energize the cellular processes.pt:void(0);" class="ref">(1) Energy conversion, photochromism, and photoelectrism are the inherent effects which are employed in many bR technical applications.pt:void(0);" class="ref">(2, 3) bR, along with the other photosensitive proteins, is not able to deal with the excess energy of photons in UV and blue spectral region and utilizes less than 0.5% of the energy from the available incident solar light for its biological function.pt:void(0);" class="ref">(4) Here, we proceed with optimization of bR functions through the engineering of a “nanoconverter” of solar energy based on semiconductor quantum dots (QDs) tagged with the PM. These nanoconverters are able to harvest light from deep-UV to the visible region and to transfer this additionally collected energy to bR via Frster resonance energy transfer (FRET). We show that specific nanobio-optical and spatial coupling of QDs (donor) and bR retinal (acceptor) provide a means to achieve FRET with efficiency approaching 100%. We have finally demonstrated that the integration of QDs within PM significantly increases the efficiency of light-driven transmembrane proton pumping, which is the main bR biological function. This new QD−PM hybrid material will have numerous optoelectronic, photonic, and photovoltaic applications based on its energy conversion, photochromism, and photoelectrism properties.p>