Hydrogel Biosensor Array Platform Indexed by Shape

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• 作者：Jason E. Meiring ; Matthew J. Schmid ; Scott M. Grayson ; Benjamin M. Rathsack ; David M. Johnson ; Romy Kirby ; Ramakrishnan Kannappan ; Kalpana Manthiram ; Benjamin Hsia ; Zachary L. Hogan ; Andrew D. Ellingt
• 刊名：Chemistry of Materials
• 出版年：2004
• 出版时间：December 28, 2004
• 年：2004
• 卷：16
• 期：26
• 页码：5574 - 5580
• 全文大小：292K
• 年卷期：v.16,no.26(December 28, 2004)
• ISSN：1520-5002

文摘

A multi-analyte sensor array platform has been developed which consists of analyte specificfeatures that are indexed by shape. The array features are batch-fabricated lithographicallyfrom poly(ethylene glycol) diacrylate hydrogel pre-polymer and can accommodate a widevariety of different sensing chemistries. Depending on the physical scale of the sensingmoiety, it is either copolymerized to the hydrogel matrix (e.g., oligonucleotides, aptamers),or it is merely physically encapsulated, an important strategy for preserving the biologicalactivity of the larger and more complex sensing elements (e.g., antibodies, proteins, cells).This three-dimensional hydrogel sensor platform has an advantage over two-dimensionalplatforms in that it offers an increased signal density, and because the array is constructedof poly(ethylene glycol), it has virtually no background noise due to nonspecific adsorptionof labeled analytes. To highlight the capabilities of this platform to make high signal-to-noise measurements using diverse sensing chemistries, two demonstrations are describedherein that illustrate the platform's efficacy in oligonucleotide sensing and cell-based sensing.