Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches

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• 作者：Merc猫 Izquierdo-Serra ; Marta Gasc贸n-Moya ; Jan J. Hirtz ; Silvia Pittolo ; Kira E. Poskanzer ; 脠ric Ferrer ; Ramon Alib茅s ; F茅lix Busqu茅 ; Rafael Yuste ; Jordi Hernando ; Pau Gorostiza
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：June 18, 2014
• 年：2014
• 卷：136
• 期：24
• 页码：8693-8701
• 全文大小：635K
• 年卷期：v.136,no.24(June 18, 2014)
• ISSN：1520-5126

文摘

Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution.