Carotenoid Biosynthetic Pathway in the Citrus Genus: Number of Copies and Phylogenetic Diversity of Seven Genes
详细信息 查看全文
- 作者：Anne-Laure Fanciullino ; Claudie Dhuique-Mayer ; Franç ; ois Luro ; Raphaë ; l Morillon ; Patrick Ollitrault
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：September 5, 2007
- 年：2007
- 卷：55
- 期：18
- 页码：7405 - 7417
- 全文大小：194K
- 年卷期：v.55,no.18(September 5, 2007)
- ISSN：1520-5118
文摘
The first objective of this paper was to analyze the potential role of allelic variability of carotenoidbiosynthetic genes in the interspecific diversity in carotenoid composition of Citrus juices. The secondobjective was to determine the number of copies for each of these genes. Seven carotenoidbiosynthetic genes were analyzed using restriction fragment length polymorphism (RFLP) and simplesequence repeats (SSR) markers. RFLP analyses were performed with the genomic DNA obtainedfrom 25 Citrus genotypes using several restriction enzymes. cDNA fragments of Psy, Pds, Zds, Lcy-b, Lcy-e, Hy-b, and Zep genes labeled with [
-32P]dCTP were used as probes. For SSR analyses,two primer pairs amplifying two SSR sequences identified from expressed sequence tags (ESTs) ofLcy-b and Hy-b genes were designed. The number of copies of the seven genes ranged from onefor Lcy-b to three for Zds. The genetic diversity revealed by RFLP and SSR profiles was in agreementwith the genetic diversity obtained from neutral molecular markers. Genetic interpretation of RFLPand SSR profiles of four genes (Psy1, Pds1, Lcy-b, and Lcy-e1) enabled us to make inferences onthe phylogenetic origin of alleles for the major commercial citrus species. Moreover, the results ofour analyses suggest that the allelic diversity observed at the locus of both of lycopene cyclase genes,Lcy-b and Lcy-e1, is associated with interspecific diversity in carotenoid accumulation in Citrus. Theinterspecific differences in carotenoid contents previously reported to be associated with other keysteps catalyzed by PSY, HY-b, and ZEP were not linked to specific alleles at the corresponding loci.
-32P]dCTP were used as probes. For SSR analyses,two primer pairs amplifying two SSR sequences identified from expressed sequence tags (ESTs) ofLcy-b and Hy-b genes were designed. The number of copies of the seven genes ranged from onefor Lcy-b to three for Zds. The genetic diversity revealed by RFLP and SSR profiles was in agreementwith the genetic diversity obtained from neutral molecular markers. Genetic interpretation of RFLPand SSR profiles of four genes (Psy1, Pds1, Lcy-b, and Lcy-e1) enabled us to make inferences onthe phylogenetic origin of alleles for the major commercial citrus species. Moreover, the results ofour analyses suggest that the allelic diversity observed at the locus of both of lycopene cyclase genes,Lcy-b and Lcy-e1, is associated with interspecific diversity in carotenoid accumulation in Citrus. Theinterspecific differences in carotenoid contents previously reported to be associated with other keysteps catalyzed by PSY, HY-b, and ZEP were not linked to specific alleles at the corresponding loci.