文摘
The DNA compacting properties of polyamines (especially spermine) are well-known, hence the use of spermineas the cationic part in several synthetic DNA carriers. Here, we describe the synthesis of modified spermines,with a "lipophosphoramidate" as the lipidic part, and their use for efficient in vitro transfection. Physicochemicalmeasurements (particle size, zeta potentials, pKa determination) and gel retardation assays were also performed.Theoretical membrane-disrupting ability was established by FRET. Taken together, our results indicate thatlipophosphoramidates constitute an interesting alternative to "classical" lipidic parts of cationic lipids used asDNA carriers.