Layer-by-Layer Self-Assembly of Chitosan and Poly(纬-glutamic acid) into Polyelectrolyte Complexes

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• 作者：Joana C. Antunes ; Catarina Leite Pereira ; Maria Molinos ; Frederico Ferreira-da-Silva ; Mariagemiliana Dessı ; ̀ ; Antonio Gloria ; Luigi Ambrosio ; Raquel M. Gon莽alves ; M谩rio A. Barbosa
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：December 12, 2011
• 年：2011
• 卷：12
• 期：12
• 页码：4183-4195
• 全文大小：542K
• 年卷期：v.12,no.12(December 12, 2011)
• ISSN：1526-4602

文摘

Chitosan (Ch) is a nontoxic and biocompatible polysaccharide extensively used in biomedical applications. Ch, as a polycation, can be combined with anionic polymers by layer-by-layer (LbL) self-assembly, giving rise to multilayered complexed architectures. These structures can be used in tissue engineering strategies, as drug delivery systems, or artificial matrices mimicking the extracellular microenvironment. In this work, Ch was combined with poly(纬-glutamic acid) (纬-PGA). 纬-PGA is a polyanion, which was microbially produced, and is known for its low immunogenic reaction and low cytotoxicity. Multilayered ultrathin films were assembled by LbL, with a maximum of six layers. The interaction between both polymers was analyzed by: ellipsometry, quartz crystal microbalance with dissipation, Fourier transform infrared spectroscopy, atomic force microscopy, and zeta potential measurements. Ch/纬-PGA polyelectrolyte multilayers (PEMs) revealed no cytotoxicity according to ISO 10993-5. Overall, this study demonstrates that Ch can interact electrostatically with 纬-PGA forming multilayered films. Furthermore, this study provides a comprehensive characterization of Ch/纬-PGA PEM structures, elucidating the contribution of each layer for the nanostructured films. These model surfaces can be useful substrates to study cell鈥揵iomaterial interactions in tissue regeneration.