Laccase from Trametes sp. I-62: Production, Characterization, and Application as a New Laccase for Eucalyptus globulus Kraft Pulp Biobleaching

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• 作者：Raquel Martin-Sampedro ; Jesus Miranda ; Juan C. Villar ; Maria E. Eugenio
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2013
• 出版时间：November 6, 2013
• 年：2013
• 卷：52
• 期：44
• 页码：15533-15540
• 全文大小：363K
• 年卷期：v.52,no.44(November 6, 2013)
• ISSN：1520-5045

文摘

The production of laccase from Trametes sp. I-62 was optimized, and then it was applied for the first time in biobleaching in order to contribute to solve some problems associated with pulp bleaching. When wheat straw and copper sulfate were used in combination as inductors, the maximum laccase activity was obtained. The resulting enzymatic fluid was applied at optimum pH and temperature jointly with acetosyringone in a pretreatment stage (L) aimed to enhance the subsequent bleaching consisting of an alkaline extraction (E) and a hydrogen peroxide stage (P). Results showed that, even when a significantly lower amount of hydrogen peroxide was consumed in the LEP sequence (41.9% vs 89.9% for EP), higher delignification (40.6% vs 33.4%) and brightness (63.6 vs 56.3% ISO) were achieved. After accelerated aging, the optical properties of the biobleached pulp were found to be less stable but still remained higher than those of control pulps.