Functional Patterning of DNA Origami by Parallel Enzymatic Modification

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• 作者：Kasper Jahn ; Thomas T酶rring ; Niels Vinther Voigt ; Rasmus Sch酶ler S酶rensen ; Anne Louise Bank Kodal ; Ebbe Sloth Andersen ; Kurt Vesterager Gothelf ; J酶rgen Kjems
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：April 20, 2011
• 年：2011
• 卷：22
• 期：4
• 页码：819-823
• 全文大小：833K
• 年卷期：v.22,no.4(April 20, 2011)
• ISSN：1520-4812

文摘

We demonstrate here a rapid and cost-effective technique for nanoscale patterning of functional molecules on the surface of a DNA origami. The pattern is created enzymatically by transferring a functionalized dideoxynucleotide to the 3鈥?end of an arbitrary selected set of synthetic DNA oligonucleotides positioned approximately 6 nm apart in a 70 脳 100 nm² rectangular DNA origami. The modifications, which are performed in a single-tube reaction, provide an origami surface modified with a variety of functional groups including chemical handles, fluorescent dyes, or ligands for subsequent binding of proteins. Efficient labeling and patterning was demonstrated by gel electrophoresis shift assays, reverse-phase HPLC, mass spectrometry, atomic force microscopy (AFM) analysis, and fluorescence measurements. The results show a very high yield of oligonucleotide labeling and incorporation in the DNA origami. This method expands the toolbox for constructing several different modified DNA origami from the same set of staple strands.