Energetically Biased DNA Motor Containing a Thermodynamically Stable Partial Strand Displacement State

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• 作者：Preston B. Landon ; Joon Lee ; Michael Taeyoung Hwang ; Alexander H. Mo ; Chen Zhang ; Anthony Neuberger ; Brian Meckes ; Jose J. Gutierrez ; Gennadi Glinsky ; Ratnesh Lal
• 刊名：Langmuir
• 出版年：2014
• 出版时间：November 25, 2014
• 年：2014
• 卷：30
• 期：46
• 页码：14073-14078
• 全文大小：357K
• ISSN：1520-5827

文摘

Current work in tuning DNA kinetics has focused on changing toehold lengths and DNA concentrations. However, kinetics can also be improved by enhancing the completion probability of the strand displacement process. Here, we execute this strategy by creating a toehold DNA motor device with the inclusion of a synthetic nucleotide, inosine, at selected sites. Furthermore, we found that the energetic bias can be tuned such that the device can stay in a stable partially displaced state. This work demonstrates the utility of energetic biases to change DNA strand displacement kinetics and introduces a complementary strategy to the existing designs.