文摘
In this work, the viability of using aqueous-phase sugars derived from the pyrolysis of lignocellulosic biomass was analyzed using high-throughput screening in microtiter plates. To standardize results, a synthetic aqueous phase of pyrolytic bio-oil was constructed based on typical constituents and composition ranges and then used to determine the fermentation viability of Saccharomyces cerevisiae. The effects of inhibitory compounds in pyrolytic bio-oil were assessed by fitting measured growth kinetics to the model of Baranyi and Roberts ( Int. J. Food Microbiol. 1994, 23, 277), specifically on the fitted growth rates, initial microorganism adaptation, and maximum biomass densities. It was found that even a dilution to approximately 10% of the hypothetical inhibitor concentration in aqueous bio-oil was significantly inhibitory to growth, although the presence of additional sugars was able to moderate this impact slightly. The high-throughput screening used in this work allowed for the rapid measurement of a variety of inhibitors at different concentrations, as well as inhibitory mixtures.