Pyrolysis Byproducts as Feedstocks for Fermentative Biofuel Production: An Evaluation of Inhibitory Compounds through a Synthetic Aqueous Phase

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• 作者：Karen Schwab ; Jeffery A. Wood ; Lars Rehmann
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2013
• 出版时间：December 26, 2013
• 年：2013
• 卷：52
• 期：51
• 页码：18234-18240
• 全文大小：335K
• ISSN：1520-5045

文摘

In this work, the viability of using aqueous-phase sugars derived from the pyrolysis of lignocellulosic biomass was analyzed using high-throughput screening in microtiter plates. To standardize results, a synthetic aqueous phase of pyrolytic bio-oil was constructed based on typical constituents and composition ranges and then used to determine the fermentation viability of Saccharomyces cerevisiae. The effects of inhibitory compounds in pyrolytic bio-oil were assessed by fitting measured growth kinetics to the model of Baranyi and Roberts ( Int. J. Food Microbiol. 1994, 23, 277), specifically on the fitted growth rates, initial microorganism adaptation, and maximum biomass densities. It was found that even a dilution to approximately 10% of the hypothetical inhibitor concentration in aqueous bio-oil was significantly inhibitory to growth, although the presence of additional sugars was able to moderate this impact slightly. The high-throughput screening used in this work allowed for the rapid measurement of a variety of inhibitors at different concentrations, as well as inhibitory mixtures.