Assembly of Alternating Polycation and DNA Multilayer Films by Electrostatic Layer-by-Layer Adsorption

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• 作者：Renjun Pei ; Xiaoqiang Cui ; Xiurong Yang ; and Erkang Wang
• 刊名：Biomacromolecules
• 出版年：2001
• 出版时间：June 2001
• 年：2001
• 卷：2
• 期：2
• 页码：463 - 468
• 全文大小：68K
• 年卷期：v.2,no.2(June 2001)
• ISSN：1526-4602

文摘

The assembly of alternating DNA and positively charged poly(dimethyldiallylammonium chloride) (PDDA)multilayer films by electrostatic layer-by-layer adsorption has been studied. The real-time surface plasmonresonance (BIAcore) technique was used to characterize and monitor the formation of multilayer films insolution in real time continuously. Electrochemical impedance spectroscopy (EIS) and UV-vis absorbancemeasurements were also used to study the film assembly, and linear film growth was observed. All theresults indicate that the uniform multilayer can be obtained on the poly(ethylenimine)- (PEI-) coated substratesurface. The kinetics of the adsorption of DNA on PDDA surface was also studied by the real-time BIAcoretechnique; the observed rate constant was calculated using a Langmuir model (k_obs = (1.28 ± 0.08) × 10^-2s^-1.