Differences in the Neuroprotective Effect of Orally Administered Virgin Olive Oil (Olea europaea) Polyphenols Tyrosol and Hydroxytyrosol in Rats
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- 作者：Jos茅 Pedro De La Cruz ; Maria Isabel Ruiz-Moreno ; Ana Guerrero ; Jos茅 Julio Reyes ; Adela Benitez-Guerrero ; Jos茅 Luis Espartero ; Jos茅 Antonio Gonz谩lez-Correa
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：July 1, 2015
- 年：2015
- 卷：63
- 期：25
- 页码：5957-5963
- 全文大小：251K
- ISSN：1520-5118
文摘
The neuroprotective effect of virgin olive oil (VOO) polyphenols has been related to their antioxidant effect. The main objective was to analyze how tyrosol and hydroxytyrosol contribute to the antioxidant and neuroprotective effects of VOO in a model of hypoxia鈥搑eoxygenation in rat brain slices. Rats were treated per os (po) (10 or 20 mg/kg/day) with hydroxytyrosol ethyl ether (HTEE), tyrosol ethyl ether (TEE), or 3,4-di-o-methylidene-hydroxytyrosol ethyl ether (MHTEE), used as a negative control for antioxidant effects. Lipid peroxidation was inhibited with HTEE, TEE, and MHTEE (from 5.0 卤 1.5 to 2.6 卤 1.5, 4.5 卤 1.5, and 4.8 卤 1.5 nmol/mg protein, respectively). However, all three compounds had similar neuroprotective effects: from 2.8 卤 0.07 to 1.8 卤 0.02 arbitrary units for HTEE, 1.4 卤 0.09 arbitrary units for TEE, and 1.3 卤 0.2 arbitrary units for MHTEE. All three compounds inhibited 3-nitrotyrosine production (from 3.7 卤 0.3 to 1.2 卤 0.03 nmol/0.1 g tissue for HTEE, 1.0 卤 0.2 nmol/0.1 g tissue for TEE, and 1.3 卤 0.1 nmol/0.1 g tissue for MHTEE), prostaglandin E2 production (from 55.7 卤 2.2 to 46.4 卤 1.9 pg/0.1 g tissue for HTEE, 24.7 卤 1.3 pg/0.1 g tissue for TEE, and 27.6 卤 2.6 pg/0.1 g tissue for MHTEE), whereas only HTEE inhibited IL1尾 production (from 35.7 卤 1.5 to 21.6 卤 0.8 pg/0.1 g tissue). Pearson correlation coefficients related neuroprotective effect with an antioxidant effect for HTEE (R = 0.72, p < 0.001), and inhibition of nitrosative stress (R = 0.78, 0.67, and 0.66 for HTEE, TEE, and MHTEE, respectively, p < 0.001) and inflammatory mediators (R = 0.72, 0.79, and 0.64 for HTEE, TEE, and MHTEE, respectively, p < 0.001) with all three compounds.