Hapten Synthesis and Monoclonal Antibody-Based Immunoassay Development for the Detection of the Fungicide Kresoxim-methyl

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• 作者：Josep V. Mercader ; Celia Su&#xe1 ; rez-Pantaleó ; n ; Consuelo Agulló ; Antonio Abad-Somovilla ; Antonio Abad-Fuentes
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：March 12, 2008
• 年：2008
• 卷：56
• 期：5
• 页码：1545 - 1552
• 全文大小：269K
• 年卷期：v.56,no.5(March 12, 2008)
• ISSN：1520-5118

文摘

Strobilurin fungicides have been increasingly used for fungus pest control since they were introduced in 1996. For pesticide residue detection, immunoassays constitute nowadays a valuable approach. This paper describes the synthesis of functionalized haptens of kresoxim-methyl, the production of monoclonal antibodies, and the development of enzyme-linked immunosorbent assays. On the one hand, a two-step conjugate-coated immunoassay was optimized using extended or short incubation times, with limits of detection of 0.4 ng/mL for the extended assay and 0.3 ng/mL for the rapid assay. On the other hand, an immunoassay was optimized following a procedure consisting of just one incubation step. This one-step assay had a limit of detection of 0.4 ng/mL. All of these assays showed a similar performance, with sensitivities well below common maximum residue limits for this pesticide (50 µg/kg) and lower than the detection limits of the usual chromatographic detection methods.