Raffin
ose
olig
osaccharides (RO) are
the fac
tors primarily resp
onsible f
or fla
tulence up
on inges
ti
on
of s
oybean-derived pr
oduc
ts. ROs are hydr
olyzed by
-galac
tosidases
tha
t cleave
-1,6-linkages
of
-galac
toside residues. The
objec
tives
of
this s
tudy were
the purifica
ti
on and charac
teriza
ti
on
ofex
tracellular
-galac
tosidase fr
om
Debaryomyces hansenii UFV-1. The enzyme purified by gel fil
tra
ti
onand ani
on exchange chr
oma
tographies presen
ted an
Mr value
of 60 kDa and
the
N-
terminal amin
oacid sequence YENGLNLVPQMGWN. The
Km values f
or hydr
olysis
of
pNP
Gal, melibi
ose, s
tachy
ose,and raffin
ose were 0.30, 2.01, 9.66, and 16 mM, respec
tively. The
-galac
tosidase presen
ted abs
olu
tespecifici
ty f
or galac
tose in
the
-p
osi
ti
on, hydr
olyzing
pNPGal, s
tachy
ose, raffin
ose, melibi
ose, andp
olymers. The enzyme was n
onc
ompe
ti
tively inhibi
ted by galac
tose (
Ki = 2.7 mM) and melibi
ose (
Ki= 1.2 mM). Enzyme
trea
tmen
ts
of s
oy milk f
or 4 h a
t 60
ti
ties/deg.gif">C reduced
the am
oun
ts
of s
tachy
ose andraffin
ose by 100%.Keyw
ords:
-Galac
tosidase;
Debaryomyces hansenii UFV-1; raffin
ose
olig
osaccharides; charac
teriza
ti
on; fla
tulence