Conformational Memory of a Protein Revealed by Single-Molecule Spectroscopy

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• 作者：Mario Sch枚rner ; Sebastian Reinhardt Beyer ; June Southall ; Richard J. Cogdell ; J眉rgen K枚hler
• 刊名：Journal of Physical Chemistry B
• 出版年：2015
• 出版时间：November 5, 2015
• 年：2015
• 卷：119
• 期：44
• 页码：13964-13970
• 全文大小：529K
• ISSN：1520-5207

文摘

Proteins are supramolecular machines that carry out a wide range of different functions, many of which require flexibility. Up until now spontaneous conformational fluctuations of proteins have always been assumed to reflect a stochastic random process. However, if changing between different conformational states was random, then it would be difficult to understand how conformational control of protein function could have evolved. Here we demonstrate that a single protein can show conformational memory. This is exactly the process that can facilitate the evolution of control of switching between two conformational states that can then be used to regulate protein function.