The Open, the Closed, and the Empty: Time-Resolved Fluorescence Spectroscopy and Computational Analysis of RC-LH1 Complexes from Rhodopseudomonas palustris

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• 作者：Sebastian R. Beyer ; Lars M眉ller ; June Southall ; Richard J. Cogdell ; G. Matthias Ullmann ; J眉rgen K枚hler
• 刊名：Journal of Physical Chemistry B
• 出版年：2015
• 出版时间：January 29, 2015
• 年：2015
• 卷：119
• 期：4
• 页码：1362-1373
• 全文大小：657K
• ISSN：1520-5207

文摘

We studied the time-resolved fluorescence of isolated RC-LH1 complexes from Rhodopseudomonas palustris as a function of the photon fluence and the repetition rate of the excitation laser. Both parameters were varied systematically over 3 orders of magnitude. On the basis of a microstate description we developed a quantitative model for RC-LH1 and obtained very good agreement between experiments and elaborate simulations based on a global master equation approach. The model allows us to predict the relative population of RC-LH1 complexes with the special pair in the neutral state or in the oxidized state P⁺ and those complexes that lack a reaction center.