Development of a Clickable Designer Monolignol for Interrogation of Lignification in Plant Cell Walls
详细信息 查看全文
- 作者：Natalie Bukowski ; Jyotsna L. Pandey ; Lucas Doyle ; Tom L. Richard ; Charles T. Anderson ; Yimin Zhu
- 刊名：Bioconjugate Chemistry
- 出版年：2014
- 出版时间：December 17, 2014
- 年：2014
- 卷：25
- 期：12
- 页码：2189-2196
- 全文大小：354K
- ISSN：1520-4812
文摘
Lignin is an abundant and essential polymer in land plants. It is a prime factor in the recalcitrance of lignocellulosic biomass to agricultural and industrial end-uses such as forage, pulp and papermaking, and biofuels. To better understand lignification at the molecular level, we are developing a lignin spectroscopic and imaging toolbox on one 鈥渘egligible鈥?auxiliary. Toward that end, we describe the design, synthesis, and characterization of a new designer monolignol, 3-O-propargylcaffeyl alcohol, which contains a bioorthogonal alkynyl functional group at the 3-O-position. Importantly, our data indicate that this monolignol does not alter the fidelity of lignification. We demonstrate that the designer monolignol provides a platform for multiple spectroscopic and imaging approaches to reveal temporal and spatial details of lignification, the knowledge of which is critical to reap the potential of energy-rich renewable plant biomass for sustainable liquid fuels and other diverse economic applications.